P-9: Protective Effect of Achillea Millefolium Extract Against Cyclosporine - Induced Toxicity on the Fertility Rate in Adult Male Rats

Background: Cyclosporine is one of the main immunosuppressors used for renal transplant recipients, and is given to prevent transplant rejection. Although the drug increases the survival of patients and grafted organs, it has some side effects independent of its effect on the immune system that are usually ignored. Achillea Millefolium is famous for its medical application as an analgesic and antiseptic in folk medicine. In this study, the effect of Achillea Millefolium extract on the embryo quality in adult male rats which were treated with cyclosporine. Materials and Methods: This in study, 24 rats were randomly divided into 3 groups as described below according to the treatment they received; control group, Achillea Millefolium + cyclosporine group(100 mg and 45 mg/ kg per day, orally respectively) cyclosporine group (45 mg/ kg per day, orally) .The treatment period was 45 days. Percentage of zygotes, two cell, blastocyct and type of embryos (type I, embryo with low quality type II embryo moderate type II high quality) were evaluated. Results: Results indicated that the percentage of zygotes or fertilized oocytes, two cell and blastocyct embryoes reduced in cyclosporine -treated rat in compartion with control and Achillea Millefolium + cyclosporine groups, also type III (high quality) embryos increased in Achillea Millefolium + cyclosporine group. Conclusion: Cyclosporine - induced toxicity on fertility caused to decrease in zygotes and level premental of embryoes. But Achillea Millefolium caused to increased of zygots and improve of development embry to Blastocyts stage.